单克隆抗体MG7在胃癌癌前病变组织中的免疫组织化学研究

An Immunohistochemical Study Using McAb MG7 on the Precancerous Lesion of Gastric Cancer

作者应用胃癌单克隆抗体MG7对289例胃粘膜活检标本进行PAP免疫组织化学观察,发现伴有肠化生的萎缩性胃炎组(20.0％)与伴有肠化生的癌旁粘膜组(62.1％)间、弥漫型胃癌癌旁粘膜肠化生组(41.7％)与肠型胃癌癌旁粘膜肠化生组(76.5％)间、轻型不典型增生组(23.9％)与中重度不典型增生组(54.0％)间,MG7-Ag表达阳性率均有显著性差异(均为P<0.01)。在胃癌组织中MG7-Ag表达阳性率为87.8％,而8例正常胃粘膜均阴性。结果表明,胃癌单克隆抗体MG7对胃癌的诊断具有较高的特异性;肠化生与胃癌(尤其是肠型胃癌)的发生有密切关系;对MG7-Ag表达阳性的肠化生和异型增生患者加强随访,将有利于胃癌的早期发现。

The biopsies of 289 cases of gastric cancer mucosa were investigated by PAP itnmunohistochemical staining of MG7, a monoclonal antibody (McAb) against human gastric cancer (GC) . The positive rate (62.1%) of MG7 stainings in intestinal metaplasia (IM) adjacent to GC was significantly higher than that (20%)from chronic atrophic gastritis (P<0.005). The positive rate (79.5%) of MG7 stainings in IM adjacent to intestinal type of GC was significantly higher than that (41.1%) adjacent to diffusetype of GC (P<0.01). The positive rate (54%) of MG7 staining in moderate and severe hyperplasia was also significantly higher than that (23.9%) in mild hyp-erplasia (P<0.005). The positive rate of MG7-Ag in GC was 87.85%, but all of the normal gastric mucosa were negative. Our results indicate that McAb MG7 is specific and sensitive to detection of GC. IM is closely related to GC, especially intestinal type of GC. McAb MG7 is of use as tumor marker and its positive reactivity has important value in screening cases with IM and hyperplasia.