反相高效液相色谱-荧光法测定人血浆中维库溴铵的浓度

Determination of Vecuronium Concentration in Human Plasma by RP-HPLC with Fluorescence Detection

目的：建立以反相高效液相色谱-荧光法测定人血浆中维库溴铵浓度的方法。方法：色谱柱为Shim-pak CLC-ODS，流动相为1，4-二氧六环-0．1mol／L磷酸二氢钠：0．11mol／L庚烷磺酸钠缓冲液（20：80），样品提取分离后用流动相溶解，在Ex／Em=380／452nm波长处检测。结果：维库溴铵检测浓度在20～200ng／ml范围内线性关系良好（r=0．9995），最低检测浓度为10ng／ml（S／N=10），绝对回收率在98．63％～99．81％之间，方法回收率在98．83％～102．13％之间，日内、日间变异系数均小于10％。结论：本方法简便、稳定、灵敏，可满足临床药动学研究的需要。

OBJECTIVE ： To establish a method for the determination of vecuronium in human plasma by RP - HPLC with fluorescence detection. METHODS： The determination was performed with Shim - pak CLC - ODS as chromatographic column. The mobile phase consisted of 1,4- dioxane - 0. 1mol/L ammonium dihydrogen phosphate ： 0.11mol/L sodium heptanesulfonate buffer solution （20 ： 80）. Vecuronium was extracted from plasma and dissolved in the mobile phase, and detected at the wavelength of 380nm（excitation） and 452nm （emission） . RESULTS： Vecuronium was linear within the concentration range of 20-200ng/ml（r = 0.9 995）. The limit of detection （LOD） was 10ng/ml （S/N = 10） . The absolute recovery was 98.63% -99.81% , and the method recovery was 98.83%- 102.13%. The intra- day and inter- day coefficients of variability were both less than 10%. CONCLUSION： The method is simple, stable and highly sensitive and applicable for the research of clinical pharmacokinetics.