高效液相色谱法测定附子中3组分的含量

Simultaneous Content Determination of Aconitine,Hypaconitine and Mesaconitine in Fuzi by HPLC

目的:建立以高效液相色谱法同时测定附子中乌头碱、新乌头碱、次乌头碱含量的方法。方法:色谱柱为HypersilC8,流动相为甲醇-0·04mol/L三乙胺(50∶50,磷酸调节pH值至6),流速为1·0ml/min,检测波长为230nm,柱温为40℃。结果:乌头碱、新乌头碱、次乌头碱进样量分别在0·0008μg^0·19μg(r=0·9996)、0·00159μg^0·40μg(r=0·9998)、0·00186μg^0·47μg(r=0·9997)范围内与峰面积积分值线性关系良好,平均回收率分别为94·2%(RSD=2·0%)、95·2%(RSD=1·3%)、96·4%(RSD=1·8%)。结论:本方法简便、准确、分离效果好、线性范围宽、灵敏度高,可用于本品的质量控制。

OBJECTIVE： To establish an HPLC method for simultaneous content determination of aconitine,mesaconitine, and hypaconitine in Fuzi. METHODS： Hypersil C8 was used as chromatographic column, methanol- 0.04mol/L triethylamine （pH adjusted to 6 with HaPO4）（50 ： 50）as mobile phase. The detection wavelength was set at 230nm,the flow rate at 1.0ml/min and the column temperature at 40℃ .RESULTS：The sample sizes of aconitine, mesaconitine, hypaconitine had good linear relationship with their peak areas in the range of 0.0 008μg-0.19μg（r=0. 9 996）, 0.00 159μg-0.40μg（r = 0.9 998）, and 0.00 186μg-0.47μg（r=0. 9 997）, respectively.Their average recoveries were 94.2% （RSD = 2.0%）, 95.2% （RSD = 1.3%）, and 96.4% （RSD=1.8% ）, respectively.CONCLUSION： The established method is convenient and accurate with satisfactory separation effect,wide linear range,and high sensitivity,and can be available for the quality control of Fuzi.