何首乌炮制工艺的研究

On Processing Technique of Radix Polygoni Multiflori

目的:优化何首乌高压炮制工艺,比较其与传统炮制工艺的差异。方法:采用正交设计,以水浸出物、二苯乙烯苷、水溶性糖含量为指标优化何首乌高压炮制工艺;比较高压炮制品与传统炮制品各指标含量、高效液相指纹图谱、不同分子量糖百分含量比的差异。结果:高压炮制何首乌影响因素大小依次为蒸制温度>蒸制时间>干燥温度;高压炮制法和传统炮制法热动力过程不同,所炮制的何首乌无论是醇溶性成分变化还是多糖分子量变化都存在差异。结论:高压炮制法能否代替传统炮制法,需药效学的进一步验证。

OBJECTIVE：To optimize the processing technique of Radix Polygoni Multiflori and to draw a comparison with the traditional processing technique.METHODS： The processing technique of Radix Polygoni Multiflori was optimized by orthogonal design with water extract, diphenyl ethylene glycoside and water-soluble saccharides as indexes.The high-pressure processed products were compared with the traditional one in parameters, HPLC fingerprints, and the percentages of saccharides of different molecular weights.RESULTS：The influential factors in high-pressure processing of radix polygoni multiflori were ranked in order as the following： steaming-temperature〉heating-time〉dry-time.The high-pressure processing and the traditional one were different in thermodynamics and the processed products by the two methods were also different either in alcohol soluble extractives or in molecular weight of polysaccharide.CONCLUSION： Further pharmacodynamic studies are needed to confirm whether it is possible for pressure - steaming processing to replace the traditional steaming processing.