摘要
我们试用标记分析法,用3种单克隆抗体(黄病毒科特异性、登革病毒属特异性和登革病毒2型型特异性单克隆抗体)研究了我国海南省1985～1987年3年内流行的登革2型病毒8个流行株的抗原性变化,并与标准株新几内亚B株进行了比较。发现6株与标准株类似,2株显示出明显的差异。标记分析法为病毒抗原分析提供了一个简便、快速的方法,可用来监测一个地区病毒株群的变化及新株的传入。
Defining the extent of antigenic variation between dengue-2 viruses from Hainan province was tried, which had been isolated over a period of three years(1985-1987). The dengue-2 viruses were compared with the prototype New Guinea B strain and were subjected to antigen signature analysis. Eight strains of dengue-2 virus were analyzed by three monoclonal antibodies, flaviviridae, dengue-virus and dengue-2 type specific reactive epitopes, over a range of antigen concentration. Six out of eight of dengue-2 virus were shown to be antigenically homogeneous, and the remaining two strains, to be heterogeneous. Signature analysis provides a rapid and simple means to differentiate strains derived from different sources, thus permiting monitoring changes or introduction of new dengue virus populations in certain geographic region.
关键词
登革热病毒
抗原
单克隆抗体
dengue virus antigen, viral
signature analysis
antibodies, monoclonal
