肾虚糖尿病家系中的血瘀证研究

Study on Blood Stasis Syndrome with the Kidney-Deficiency of Diabetes Pedigree

目的:通过一个肾虚糖尿病家系,研究血瘀证候的分子生物学基础,提示利用家系和基因芯片深入研究血瘀证分子机理的可行性。方法:对一个糖尿病家系18个成员做理化测试及肾虚血瘀诊断后,精选其中3例肾虚血瘀证糖尿病患者与该家系中的2例正常人作14000点的基因芯片杂交测试。结果:获得肾虚血瘀证糖尿病家系图谱和肾虚、血瘀症状分值比较表,同时得到肾虚血瘀证糖尿病患者与正常人的差异基因446条,尤其是血栓素A合成酶1等5条基因与血瘀证的发生密切相关。结论:利用家系研究寻找宏观的证候和微观的差异基因表达具有极强的的优势,有助于探讨肾虚血瘀糖尿病证候与差异表达基因之间的关系。

Objective： To study Blood Stasis Syndrome by molecular- biology in kidneydeficiency of diabetes pedigree. Methods： 18 members in diabetes pedigree were closed to make routine physical ＆ chemical test and the macroscopic diagnosis of kidney- deficiency and blood stasis, 3 diabetics with blood stasis syndrome and 2 healthy people in the same family, were selected to make 14000 genes chips hybrid test. Results： The pedigree maps and the comparative syndrome score tables of kidneydeficiency and blood stasis syndrome were obtained, and the 446 genes of different expressions, in particular, 5 genes such as the TBXAS1, which was used to illustrate the possibility of effects on the blood stasis were also selected Conclusion： There was an exceeding preponderance of pedigree research method, which was used to search the macroscopic syndrome and the microscopic expressions of different genes. And it would help us to understand the relationship between the syndrome of kidney - deficiency ＆ blood stasis diabetes and the genes of different expressions.