流式细胞术分析p21^(HBsAg/HBsAg)转基因小鼠肝细胞凋亡

Study on Apoptosis of the Hepatocytes in p21^(HBsAg/HBsAg) Transgenic Mice with Flow Cytometry

目的观察p21HBsAg/HBsAg转基因小鼠肝细胞凋亡。方法用流式细胞仪检测6、12、18、24月龄p21HBsAg/HBsAg转基因小鼠和p21+/+野生型小鼠新鲜肝脏组织。结果转基因小鼠肝细胞凋亡率低于同月龄野生型小鼠。结论HBsAg基因的转入和表达,抑制转基因小鼠肝细胞凋亡。

Objective To observe the apoptosis in the livers of p21^HBsAg/HBsAg transgenic mice. Methods The liver of p21^HBsAg/HBsAg transgenic mice （6, 12, 18, 24 months） and corresponding p21^＋/＋ mice were assessed by flow cytometry. Result The apoptosis rate of the hepatocytes in p21^HBsAg/HBsAg transgenic mice was lower than that of wild mice of the same months. Conclusion HBsAg can inhibit apoptosis in liver cells of p21^HBsAg/HBsAg transgenic mice.