杜仲种子的多倍体诱导研究

Studies on Polyploid Induction of Eucommia ulmoides olive

目的:改良杜仲现有品种,增加其药用成分含量,丰富遗传育种资源。方法:采用不同浓度的秋水仙素溶液对杜仲种子进行不同时间的诱导。结果:经植物形态学和染色体数检测,诱导后的材料具有明显的多倍体特征。结论:杜仲种子用0.4%的秋水仙素溶液处理48h和用浓度为0.8%的秋水仙素溶液处理24h,其诱导率都较高。但从降低生产成本来考虑,我们建议在对杜仲种子进行多倍体诱导时应选择0.4%的秋水仙素溶液诱导48h。

Objective： This experiment aimed at improving the existence breed of Eucommia ulmoides olive, enrich its inheritencable breed resource, increase its medicinal composing.Method：Use colchicines of different densities to mutagenize Eucommia ulmoides olive in different phases. Result： Eucommia ulmoides olive show obvious characters of polyploid by plant morphology and quantity of chromosome examine. Conclusion： Eucommia ulmoides olive mutagenized by colchicines density of 0.4 % for 48 h and density of 0.8 % for 24 h are more easily to be mutagenize.Considering from reducing producing cost, mutagenized by colchicines density of 0.4 % for 48 h is suggested.