摘要
60只健康雏鸡随机分为A、B、C、D四组,每组15只。9日龄时,C、D两组人工感染传染性法氏囊炎病毒(IBDV),复制动物免疫抑制模型;B、D组同时饲喂中药;A组设为对照组。结果表明:免疫抑制的D组和A组在给药3 d后淋巴细胞转化率显著高于C组(P<0.01);喂药7 d后,B组转化率显著高于A组(P<0.05);中药合剂直接刺激A、C组的淋巴细胞,中药浓度为1:1000细胞转化率最高,显著高于0、1:100、1:500(P<0.01),略高于1:2000(P<0.05);ELISA检测中,D组IL-2水平显著高于C组(P<0.01),但与A组差异不显著(P>0.05)。实验提示该中药合剂可有效缓解免疫抑制病,增强机体免疫力。
60 healthy chickens were used in the study,which were divided randomly into A,B,C and D four groups, 15 in each group. On the 9th day, C and D groups were infected IBDV artificially. So the immunosuppression model was established. At the same time, B and D were fed with Chinese herbs. A group was the control one. The 3-day result indicated that lymphocyte transformation in group D and group A was greatly higher than group C （P〈0.01）. After 7 days, the transformation of group B was increased. When using the Chinese herbs to impulse the lymphocyte of A and C groups in the cell culture, the transformation was very high at 1 ： 1000 of herb concentration. The examination of IL-2 quantity showed that D group was obviously higher than C （P〈0.01）, but almost the same as group A （P〈0.05）. So this medcine can effectively relax immunosuppression and enhance immunity.
出处
《家畜生态学报》
2006年第4期98-100,共3页
Journal of Domestic Animal Ecology
基金
山东省科技攻关计划项目(2001)
