雄黄纳米微粒对人白血病细胞株HL-60的诱导分化作用

Differentiation of HL-60 cells induced by realgar nano-particles

目的：探讨雄黄（As4S4）纳米微粒对于人急性早幼粒白血病细胞HL-60的抑制增殖和诱导分化作用。方法：采用MTT法观察低浓度雄黄对HL-60细胞增殖的影响，Wright-Giemsa染色观察细胞形态，硝基四氮唑蓝（NBT）还原反应和流式细胞术抗原检测等鉴定细胞的分化。结果：细胞经雄黄处理后呈现明显的分化形态。经0．25-1．0μmol·L^-1雄黄作用24h后，NBT阳性率升高。用0．50μmol·L^-1雄黄处理48h，细胞表面分化抗原CD11b表达升高，细胞周期阻滞于G期。结论：低浓度雄黄对HL-60细胞具有诱导分化作用。

Objective. To investigate the proliferation inhibition and the differentiation effects of realgar （As4S4） nano-particles on human acute myeloid leukemia cell line HL-60. Method： Cell viability was determined by MTT and PI-stained cell cycle assays. The realgar induced morphological changes on cells were examined after Wright-Giemsa staining. The cell differentiation was evaluated with NBT and specific cell surface antigen （CDllb and CD14） expression assays. Result： HL-60 cells exhibited obvious morphological features of differentiation after the realgar treatment. A 24 h incubation of the cells with 0.25-1.0μmol·L^-1 realgar caused a great increase in NBT reduction ability. The expressious of CD11b and CD14 were augmented in cells treated with 0.50μmol·L^-1 realgar for 48 h, and cell cycles were arrested in G1 phase. Conclusion： Low dose realgar induces differentiation in human acute myeloid leukemia cell line HL-60.