摘要
目的:探讨雄黄(As4S4)纳米微粒对于人急性早幼粒白血病细胞HL-60的抑制增殖和诱导分化作用。方法:采用MTT法观察低浓度雄黄对HL-60细胞增殖的影响,Wright-Giemsa染色观察细胞形态,硝基四氮唑蓝(NBT)还原反应和流式细胞术抗原检测等鉴定细胞的分化。结果:细胞经雄黄处理后呈现明显的分化形态。经0.25-1.0μmol·L^-1雄黄作用24h后,NBT阳性率升高。用0.50μmol·L^-1雄黄处理48h,细胞表面分化抗原CD11b表达升高,细胞周期阻滞于G期。结论:低浓度雄黄对HL-60细胞具有诱导分化作用。
Objective. To investigate the proliferation inhibition and the differentiation effects of realgar (As4S4) nano-particles on human acute myeloid leukemia cell line HL-60. Method: Cell viability was determined by MTT and PI-stained cell cycle assays. The realgar induced morphological changes on cells were examined after Wright-Giemsa staining. The cell differentiation was evaluated with NBT and specific cell surface antigen (CDllb and CD14) expression assays. Result: HL-60 cells exhibited obvious morphological features of differentiation after the realgar treatment. A 24 h incubation of the cells with 0.25-1.0μmol·L^-1 realgar caused a great increase in NBT reduction ability. The expressious of CD11b and CD14 were augmented in cells treated with 0.50μmol·L^-1 realgar for 48 h, and cell cycles were arrested in G1 phase. Conclusion: Low dose realgar induces differentiation in human acute myeloid leukemia cell line HL-60.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2006年第16期1343-1346,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金资助项目(2027100520571006)