P57^(kip2)与Ki67表达对结肠癌侵袭性的影响

Influence of P57^(kip2) and Ki67 expression on invasive properties of colon adenocarcinoma

目的：研究结肠癌中P57^(kip2)与Ki67基因的表达与肿瘤的发生、发展的关系。方法：收集69例结肠癌手术切除标本,病理诊断均为结肠癌,以10例正常结肠黏膜为实验对照。采用免疫组化技术ABC法检测P57^(kip2)与Ki67抗原在正常结肠黏膜组织和结肠癌组织中的表达。结果：结肠癌组织中P57^(kip2)抗原表达的标志指数(labeling index,LI)较正常结肠黏膜组织明显降低(P=0.0001)；Ki67抗原表达的LI较正常结肠黏膜组织明显升高(P=0.0001)；不同性别、年龄结肠癌患者癌组织中P57^(kip2)、Ki67的表达没有统计学差异；P57^(kip2)在结肠癌低分化腺癌组织中的表达较中高分化腺癌组织中的表达明显降低(P=0.032)。Ki67在结肠癌低分化腺癌组织中的表达较在中高分化腺癌组织的表达明显升高(P=0.0226)。P57^(kip2)在有淋巴管浸润的结肠癌组织中的表达较在无淋巴管浸润的结肠癌组织中的表达明显降低(P=0.0001)；Ki67在有淋巴管浸润的结肠癌组织中的表达较在无淋巴管浸润的结肠癌组织中的表达明显升高(P=0.0001)；P57^(kip2)在结肠癌Dukes分期A期和B期的癌组织中的表达较在C期和D期癌组织中的表达明显升高(P=0.0002)。Ki67在DukesA期和B期的癌组织中的表达较在C期和D期癌组织中的表达明显降低(P=0.0006)；P57^(kip2)与Ki67在结肠癌组织中的表达呈现明显的负相关,相关系数r= =0.847,P=0.000l。结论：P57^(kip2)表达下调,Ki67表达上调在结肠癌的发生、发展和转移过程中发挥着重要作用。

Objective：To investigate whether expressions of P57^kip2 and Ki67 were correlated with genesis and progression of human colon cancer. Methods：We collected 69 specimens with colon cancer tissue confirmed by pathological examination. Ten normal colon tissues were used as control. The expressions of P57^kip2 and Ki67 were examined by immunohistochemistry using avidin-biotin complex method. Results： The labeling index （LI） of the expression of P57^kip2 in the colon cancer tissue was significantly lower than that in normal colon tissue（P=0.0001） ; the LI of the expression of Ki67 in the colon cancer tissue was significantly higher than that in normal colon tissue（P=0.0001）. The age and sex of patients had no effects on the expression of P57^kip2 and Ki67 in the colon cancer tissues（P〉0.05）. The expression of P57^kip2 in poor-differentiated colon cancer tissue was significantly lower than that in the highly-differentiated colon cancer tissues （P=0.032） ; the expression of Ki67 in the poor-differentiated colon cancer tissue was significantly higher than that in the well-differentiated colon cancer tissues （P=0.0226）. The expression of P57^kip2 in the colon cancer tissue with lymphatic invasion was significantly lower than that in the colon cancer tissue without lymphatic invasion （P=0.0001） ; the expression of Ki67 in the colon cancer tissue with lymphatic invasion was significantly higher than that in the colon cancer tissue without lymphatic invasion （P=0.0001）. In the colon cancer tissue, the expression of P57^kip2 in the Dukes stage A＋B was significantly higher than that in the Dukes stage C＋D （P=0.0002） ; the expression of Ki67 in the Dukes stage A＋B was significantly lower than that in the Dukes stage C＋D（P=0.0006）. The correlation analysis showed that the expression of P57^kip2 was negatively correlated with the expression of Ki67 in the colon cancer tissue （r=0. 847, P=0. 0001）. Conclusion：The down-regulation of P57^kip2 and/or up-regulation of Ki67 play an important role in the genesis, progression and metastasis of colon adenocarcinoma.