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免疫PCR技术及其应用研究进展 被引量：9

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摘要 免疫PCR相对于普通ELISA具有明显的改进:灵敏度增加;可以同时进行多种抗原/半抗原检测;有更宽的线性范围;在和实时PCR(real-time PCR)技术结合后具有更加广阔的应用前景。抗体和DNA的交联、扩增DNA的检测、不同抗原/半抗原超灵敏检测分析中本底信号的扣除是影响免疫PCR方法成功建立的三个关键因素,而这三个因素又对应于免疫PCR系统的四个子系统,即抗原-抗体反应、桥联、指示和检测系统。该文从这四个子系统对免疫PCR技术的研究进展作一综述。
作者 黄杰(综述) 李金明(审校)
机构地区 卫生部北京医院
出处 《国际检验医学杂志》 CAS 2006年第8期709-712,共4页 International Journal of Laboratory Medicine
关键词 聚合酶链反应 酶联免疫吸附测定 抗原-抗体反应
分类号 R446.62 [医药卫生—诊断学] R753.2 [医药卫生—皮肤病学与性病学]
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参考文献16

  • 1McKie A,Samuel D,Cohen B,et al.Development of a quantitative immuno-PCR assay and its use to detect mumps-specific IgG in serum.J Immunol Methods,2002,261(1-2):167-175.
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二级参考文献9

  • 1[1]Delpech A, Gilbert D, Daliphard S, et al. Antibodies to Sm, RNP and ss-B Detected by Solid-phase ELISA Using Recombinant Antigens. a Comparison Study with Counter Immunoelectrophoresis and Immunoblotting [ J ]. J Clin Lab Anal, 1993,7:197.
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共引文献5

同被引文献111

引证文献9

二级引证文献28

国际检验医学杂志
2006年 第8期

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