化学预处理对玉米秸秆酶解糖化效果的影响

Effect of Chemical Pretreatment on Enzymatic Saccharification of Maize Straw

研究了化学预处理对玉米秸秆酶解糖化效果的影响。结果表明效果最好的是40目玉米秸秆粉用0·5%的稀盐酸在121℃预处理60min后,加入纤维素酶2.5g·(100g)-1和木聚糖酶液2mL·(100g)-1,在pH4.8的乙酸-乙酸钠缓冲液体系中,液固比为10、50℃下糖化48h,玉米秸秆的总糖产率达48.5%、纤维素和半纤维素的转化率达80.8%。

The effect of pretreatment on enzymatic saccharification of maize straw by several chemical substances were studied. The result showed the optimum conditions as follows：the maize straw powder of 40 mesh was pretreated in 0.5% HCl at 121℃ for 60 min,and then was saccarified for 48 h at 50℃ in the buffer solution of acetic acid-sodium acetate of pH 4.8, with the ratio of liquid to solid 10,appending cellulase 2.5 g . （100 g）^-1 and xylanase 2 mL .（100 g）^-1. As a result,the gross saccharification rate reached 48.5% and the transformation rate of cellulose and hemicellulose reached 80.8%.