辛芷鼻敏胶囊对变应性鼻炎大鼠模型P物质和组胺的影响

Effect of Xinzhi Bimin capsule on substance P and histamine in the naso mucosa of experimental allergic rhinitis in rat

目的:探讨辛芷鼻敏胶囊对实验大鼠变应性鼻炎模型鼻粘膜组织P物质及组胺的影响,明确其治疗变应性鼻炎的具体环节。方法:Wistar大鼠40只,随机分为空白对照组、模型组、药物对照组、实验组,每组10只,除空白对照组外,其余3组采用卵清蛋白法建立变应性鼻炎大鼠模型,然后给药物对照组和实验组大鼠灌服阳性药物和辛芷鼻敏胶囊,用免疫组化方法半定量检测各组大鼠鼻粘膜组织中P物质的阳性率及采用高效液象色谱分析方法定量测定各组大鼠组胺含量,并进行比较。结果:(1)行为学改变:模型组症状得分明显高于实验组、药物对照组和空白对照组,其差异有统计学意义(P均<0.01);(2)实验组的P物质阳性率明显低于模型组(P<0.05)与药物对照组;(3)实验组与模型组、药物对照组组胺含量差异均有统计学差异(P<0.01)。结论:辛芷鼻敏胶囊可有效降低变应性鼻炎大鼠鼻粘膜中P物质及组胺的含量,达到治疗变应性鼻炎的目的。

Objectives： To study the effect and correlation of Xinzhi Bimin capsule on expression of substance P and histamine in the naso mucosa of experimental allergic rhinitis （AR） in rat,and identify the mechanism of pharmacodynamic action. Methods： The expression of positive cell of substance P was detected in semiquantity by immunochemistry and the expression of histamine was detected in quantity by high-efficient liquid,which were compared with the different model rat established in AR and the normal control group. Results： The behavior science score of model group （7.4±0.97） was higer than that in the experimental group （1.3±0.48）, positive control group （2.9±0.56） and normal control group （0.8±0.63） （P 〈0.01）. The expressions of the proportions of positive cell to substance P and histamine were lower in the experimental group than in the model group （P 〈0.05）and the positive control group except the normal control group. Conclusions： The result suggests that the Xinzhi Bimin capsule can reduce the infiltration of EOS and treat the symptom of AR in rat by down-regulating the activation of substance P and histamine.