福建水华微囊藻毒素主要亚型及其亚慢性肝毒性

Main Subtype and its Subchronic Hepatotoxicity in Microcystins from Blooms in Fujian

目的鉴定福建省某水库水华微囊藻毒素的主要亚型,研究该毒素对大鼠的亚慢性肝毒性。方法采集微囊藻水华,固相萃取法提取微囊藻毒素,以微囊藻毒素LR和RR纯毒素为标准,高效液相色谱法测定其亚型;SD大鼠0.42μg/(kgbw)MC-LR亚慢性染毒35d,观察血清酶学和组织病理学改变。结果该微囊藻水华检测出MC-LR,未检测出MC-RR;用藻细胞裂解液〔相当于0.42μg/(kgbw))MC-LR〕亚慢性染毒大鼠,血清SOD活性显著降低,SDH水平显著升高,肝脏系数显著增大,肝组织出现炎症样变化。结论福建某水库水华微囊藻毒素的主要亚型为MC-LR;亚慢性接触0.42μg/(kgbw)MC-LR的微囊藻毒素可引起肝损伤。

Objective To study the subtype and its subchronic hepatotoxicity in microcystins from microcystis blooms in Fujian. Methods Microcystis blooms were collected and microcystins were extracted from solid phase. The microcystin - LR and micro- cystin- RR concentration in microcystis extract were detected by high performance liquid chromatography （HPLC） .Spraue-Darnley rats were administered intrapefitoneally injected with 0.42 μg/ （kg bw） microcystin- LR.The level of ALT, AS T, SDH, SOD, MDA in serum was tested and H. E staining of liver tissue was observed. Results The microcystin- LR was detected and microcystin - RR was not detected.The liver coefficent and the level of SDH were significantly higher while SOD was significantly lower in MCE group than in control group. Conclusion Microcystin - LR is the main subtype. Rats exposed to 0.42 μg/ （kg bw） microcystin - LR Can induced chronic hepatotoxicity.