摘要
目的：探讨中国浙江地区汉族人群中IFN-γ基因+874位点多态性分布,分析其与大肠癌的相关性.方法：应用突变特异性扩增系统(ARMS)- PCR对健康者132例和大肠癌患者92例IFN-γ基因+874位点多态性进行分析,并将PCR产物克隆及测序鉴定.结果：大肠癌患者IFN-γ+874位点AA基因型频率为70.6%,显著高于正常人中的57.6% (P＜0.05).进一步分析显示IFN-γ+874位点AA基因型与患者的年龄、性别、肿瘤部位、Dukes分期无关.结论：IFN-γ+874位点AA基因型可能与大肠癌的发生相关.
AIM： To investigate whether the single nucleotide polymorphism （SNP） of interferongamma （IFN-γ） ＋874 T/A correlates with colorectal cancer in Han Chinese of Zhejiang province, China.
METHODS： The alleles of IFN-γ gene were detected in 132 healthy subjects and 92 patients with colorectal cancer using amplification refractory mutation system （ARMS）-PCR. Meanwhile, the PCR products were cloned and sequenced.
RESULTS： The frequency of IFN-γ, ＋874 AA genotype was significantly higher in patients with colorectal cancer than that in the healthy individuals （70.6% vs 57.6%, P 〈 0.05）, but it had no significant correlation with the age, gender, tumor location or Dukes grading in patients with colorectal cancer （P 〉 0.05）.
CONCLUSION： IFN-γ ＋874 AA genotype may be associated with the susceptibility to colorectal cancer in Han Chinese of Zhejiang province.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第20期2022-2025,共4页
World Chinese Journal of Digestology
基金
浙江省卫生厅科研基金项目
No.97420
关键词
干扰素
大肠肿瘤
多态性
Interferon-gamma
Colorectal neoplasm
Single nucleotide polymorphism
