姜黄素对慢性低氧高二氧化碳大鼠肺动脉高压及肺动脉管壁胶原的影响

EFFECT OF CURCUMIN ON PULMONARY HYPERTENSION AND WALL COLLAGEN OF PULMONARY ARTERIOLES OF CHRONIC HYPOXIC HYPERCAPNIC RATS

目的:研究姜黄素对慢性低氧高二氧化碳大鼠肺动脉压力及肺动脉管壁I型胶原的影响。方法:36只SD大鼠随机分为正常对照组(NC组),低O2高CO24周组(HH组),低O2高CO24周+姜黄素组(HC组),采用免疫组化、图像分析等方法观察姜黄素对慢性低O2高CO2大鼠肺动脉压力、肺细小动脉显微和超微结构及肺动脉管壁Ⅰ型胶原的影响。结果:①血流动力学检测显示HH组mPAP明显高于NC组(P<0.01),HC组mPAP明显低于HH组(P<0.01),三组间mCAP无明显差异(P>0.05);②光镜下,肺细小动脉管壁面积/管总面积比值(WA/TA)、肺细小动脉中膜平滑肌细胞核密度(SMC)、肺细小动脉中膜厚度(PAMT)HH组较NC组明显增高(均P<0.01),HC组WA/TA、SMC和PAMT较HH组明显降低(均P<0.01);③电镜下,HH组肺细小动脉中膜平滑肌细胞增生,面积增大,染色质增多,外膜胶原纤维密集,HC组大鼠肺细小动脉内皮细胞结构基本正常,胶原少见,中膜平滑肌细胞和外膜胶原纤维增生较HH组明显为轻;④免疫组化法发现肺细小动脉Ⅰ型胶原平均吸光度值HH组明显高于NC组(P<0.01),HC组明显低于HH组(P<0.01)。结论:姜黄素具有降低慢性低O2高CO2性肺动脉高压、改善肺血管重建及抑制肺动脉管壁I型胶原沉积的作用。

Aim： To study the effect of curcumin on pulmonary arterial pressure and type Ⅰ collagen of pulmonary arterioles in pulmonary hypertensive rats induced by chronic hypoxia and hypercapnia. Methods： Thirty six rats were randomly divided into three groups, normal control group （NC）, hypoxic hypercapnic group （HH） and hypoxic hypercapnia ＋ curcumin group （HC）. Collagen Ⅰ in pulmonary arterioles was observed by the technique of immunohistochemistry. Results. ① The findings from hemodynamics showed that the mPAP in group HH was significantly higher than that in group NC and HC. Differences of mCAP among groups were not significant （ P 〉 0.05）. ② Light microscopy showed the value of WA/TA （vessel wall area/total area）, SMC（ the density of medial smooth muscle cells） and thickness of pulmonary arterial media smooth cell layer（PAMT） were significantly higher in group HH than group NC（ P 〈 0.01 ） and group HC（ P 〈 0.01 ）. ③ Electron microscopy showed that structure of the endothelial cells in pulmonary arterioles in group HC was near to normal , and the proliferation of medial smooth muscle cells and collagen fibers in adventitia was much lighter than those of group HH. ④Expression of collagen Ⅰ in pulmonary arterioles was significantly higher in group HH than group NC（ P 〈 0.01 ） and group HC（ P 〈 0.01 ）. Conclusion ： Curcumin can decrease pulmonary arterial pressure, improve pulmonary vessel remodeling and inhibit the deposition of collagen Ⅰ in pulmonary arterioles.