摘要
目的:研究转化生长因子β1(TGF-β1)及其下游Smad3信号蛋白在大鼠心肌细胞肥大中的作用。方法:TGF-β1干预培养新生大鼠心肌细胞,流式细胞仪检测心肌细胞总蛋白含量。结扎大鼠腹主动脉复制心肌肥厚模型,在不同时间点处死动物,检测左室质量指数(LVMl),RT-PCR检测TGF-β1及Smad3的mRNA表达,Western-blot检测Smad3蛋白的表达。结果:不同剂量TGF-β1均能明显增加体外培养的心肌细胞总蛋白含量,TGF-β1(3ng/ml)还增加心肌细胞Smad3 mRNA和蛋白的表达,其表达量1 h达高峰,持续至TGF-β1刺激后8 h。大鼠腹主动脉结扎术后3 d LVMI开始上升并持续至术后28 d,心肌组织中TGF-β1、Smad3的mRNA表达水平以及Smad3蛋白表达术后3 d也开始上升持续至术后28 d,术后14 d为表达高峰(P<0.01)。结论:TGF-β1能诱导大鼠心肌细胞肥大,其信号蛋白Smad3参与了大鼠心肌肥大的病理过程。
Aim：To investigate the effects of transforming growth factor-β1 （TGF-β1 ）and singal protein Smad3 on rat myocardial hypertrophy Methods： The total protein was analysed by flow cytometer assay to judge the hypertrophy of myocardial cell incubated with diferent level of TGF-β1 in cultured myocardial cells of neonatal rats. The models of rat cardiac hypertrophy were produced with constriction of the abdominal aorta. At the difietent time after the operation, the rats were killed, and the left vertricular mass indexes （LVMl）were investigated. The mRNA expressions of TGF-β1 and Smad3 of cultured ceils and hypertrophic left ventricles were assessed by RT-PCR, the protein expressions of Smad3 were assessed by Western blot. Results： In cultured neonatal myocardial cells, difierent level TGF-β1 could significantly increase the total protein,and TGF-β1 （3ng/ml）could increase the expression of mRNA and protein of Smad3 and continued for 8 h of cultured cardiomyocytes. The LVMI and the expression of TGF-β1 mRNA and Smad3 mR- NA/protein of hypertrophic left ventricle were increased at the 3rd day after the operation and continued for 4 weeks. The peak expression of them was in 2 weeks after opration. Conclusion： TGF-β1 has the efiects on rat myocardial hypertrophy, signal protein Smad3 is included in the pathologic progress of rat myocardial hypertrophy
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2006年第3期283-287,共5页
Chinese Journal of Applied Physiology
