尾加压素Ⅱ对人脐静脉内皮细胞iNOS表达的影响

EFFECT OF UROTENSINⅡON iNOS EXPRESSION IN HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS

目的：探讨尾加压素Ⅱ（UⅡ）对人脐静脉内皮细胞（HUVEC）诱导型一氧化氮舍酶（iNOS）mRNA表达及一氧化氮（NO）合成的影响。方法：用不同浓度的UⅡ（10^-9～10^-7mol／L）干预体外培养的HUVEC，用硝酸酶还原法及比色法检测细胞培养上清液中NO的水平及iNOS的活性，半定量逆转录一聚合酶联反应（RT—PCR）法检测内皮细胞iNOSmRNA的表达。结果：UⅡ干预24h后，与空白对照组相比，UⅡ呈浓度依赖性显著刺激NO的合成（P〈0．05），增加iNOS的活性（P〈0．05），上调iNOSmRNA的表达（P〈0．05）。结论：UⅡ能刺激HUVEC的iN—OSmRNA的表达和NO的合成，提示UⅡ可能通过激活iNOS／NO途径而发挥舒张血管的作用。

Aim： To observe the direct effect of urotensin Ⅱ （U Ⅱ ） on the release of nitric oxide（NO） and expression of inducible nitric oxide synthase（iNOS） mRNA in human umbilical vein endothelial cells（HUVEC）. Methods： HUVEC were cultured with different concentrations of U Ⅱ （ 10 ^-9 - 10^ - 7mol/L） for 24 hours. Then the supematant was collected to detect the level of NO and the activity of iNOS, the expression of iNOSmRNA of HUVEC was measured by semi-quantitative reverse transcription polymerase chain reaction （RT-PCR）. Results： In comparison with controls,the level of NO, the activity of iNOS and the iNOS mRNA expression increased significantly（ P 〈 0.05 ）. Conclusion. U Ⅱ may up-regulate the expression of iNOSmRNA and increase NO generation in HUVEC, it suggests that U Ⅱ may relax blood vessel by activating iNOS/NO pathway.