表皮生长因子受体在小鼠磨牙牙胚发育过程中的表达及其生物学意义

Immunolocalization of Epidemal Growth Factor Receptor during Mouse Molar Development.

目的:探讨表皮生长因子受体在小鼠磨牙牙胚发育过程中的生物学作用。方法:用免疫组织化学检测表皮生长因子受体(EGFR)在小鼠下颌第一磨牙牙胚发育过程中的定位。结果:证实EGFR在鼠牙胚不同发育阶段呈时间-空间特异性分布模式。EGFR特别地在细胞增殖活跃的部位强染,在形态发生和分化晚期染色减弱。结论:EGF及其受体可能在调控蕾状期和帽状期形态发生的上皮-间充质相互作用中以旁分泌模式起作用。

Objective： To study the biological roles of epidemal growth factor receptor （EGFR） during mouse molar development. Methods： Immunohistochemistry was used to examine the immunolocalization of EGFR during mouse first lower molar development. Results： The results showed that EGFR was immunolocalized during mouse molar development in a temporally and spatially regulated fashion. EGFR was especially stong stained where cell actively proliferated and stained weakly during late stage of tooth morphogenesis and differentiation. Conclusion： EGFR is suggested to be involved in a paracrine fashion in the epithelial - mesenchymal interactions regulating morphogenesis of bud - and cap - stage teeth.