同源异盒基因BP_1在肺癌组织中的表达及临床意义

Clinincopathological significance of homeobox BP_1 mRNA expression in lung cancer tissue

目的本研究通过对BP1mRNA在肺癌组织中的表达状况的研究,探讨BP1mRNA在肺癌中的表达水平以及和肺癌的临床关系。方法采用逆转录聚合酶链反应(RT-PCR)技术,检测46例肺癌病人的癌组织,相应的癌旁组织和正常组织标本中BP1mRNA的表达程度及意义。结果在46组标本中,肺癌组织中有36例BP1mRNA呈阳性表达(78.26%),癌旁组织6例呈阳性表达(13.04%),正常组织中未检出阳性表达。癌组织,癌旁组织和正常组织中BP1mRNA的表达率之间存在显著性差异(P<0.01)。癌旁组织和正常组织中BP1基因的表达率之间无显著性差异(P>0.05)。在21例高分化标本中13例呈中高表达,25例低分化标本中22例呈中高表达,两者之间存在显著性差异(P<0.01);在20例Ⅰ-Ⅱ期肺癌标本中12例BP1基因呈中高表达,26例Ⅲ-Ⅳ期标本中15例呈中高表达,两者之间无显著性差异(P>0.05)。BP1基因的表达程度在24例鳞癌中21例呈高表达,在22例腺癌中19例呈高表达,两者之间无显著性差异(P>0.05)。结论BP1mRNA在肺癌组织中的表达显著上调;BP1基因的表达可能与肺癌分化程度有关,与病理类型和TNM分期无关。

Objective To investigate the expression of beta-protein 1 （BP1） gene, a novel member ofDLX homeobox gene family, in lung cancer tissue and its relationship with clinical features of lung cancer. Methods RT-PCR was employed for detecting BP1 gene expression in the lung cancer tissues, adjacent tissues, non-cancer lung tissues of 46 lung cancer patients. Results Thirty-six lung cancer tissues and 6 adjacent tissues but none of the normal tissues were found to have BP1 gene overexpression, showing significant difference in BP1 expression between the tissues （P〈0.01）. Significant difference in BP1 gene overexpression was noted between well differentiated cancers （13 of out 21） and poorly differentiated cancers （22 of the 25）, but not between cancers of different stages or between squamous carcinoma and adenocarcinoma. Conclusion BP1 gene expression is up-regulated in human lung cancer in related to the differentiation level of lung cancer but not to the clinical stage.