上皮钙粘素和β-连环蛋白在皮肤Bowen病、鳞癌中的表达

Expression of E-cadherin and beta-catenin in Bowen's disease and squamous cell carcinoma

目的研究上皮钙粘素(E-cadherin)和β-连环蛋白(β-catenin)在皮肤Bowen病(BD)、鳞癌(SCC)中的表达情况。方法免疫组化法检测15例正常皮肤、28例BD、18例SCC标本中E-cadherin和β-catenin蛋白的表达,根据染色强度、阳性细胞比率和胞质染色对结果进行半定量分析。结果与正常皮肤相比,E-cadherin和β-catenin在BD和SCC肿瘤细胞膜上的表达显著降低,而在SCC和BD肿瘤细胞的胞质或胞核β-catenin表达显著升高。结论E-cadherin/catenin复合体的异常表达在BD和SCC中常见。

Objective To investigate the involvement of E-cadherin-catenin adhesion system in Bowen＇s disease （BD） and cutaneous squamous cell carcinoma （SCC）. Methods Fifteen normal skin, 28 BD and 18 SCC specimens were stained with monoclonal antibodies against E-cadherin and beta-catenin. Evaluation of the staining results was performed with semi-quantification of the pattern and intensity of staining, percentage of positive cells, and cytoplasmic staining. Result Normal skins strongly expressed membranous E-cadherin and beta-catenin, but their expression was remarkably reduced in BD and SCC. Abnormal staining of beta-catenin was observed in the cytoplasm or cell nuclei of BD and SCC. Conclusion Abnormal expression of the E-cadherin/catenin complex is common in SCC and BD.