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nifA突变的苜蓿根瘤菌在根瘤中的转录组学分析 被引量：3

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摘要 用全基因组微阵列比较了根瘤中苜蓿根瘤菌1021的nifA突变菌和野生菌的基因表达谱.分析结果表明,nifA的缺失引起601个基因的表达发生变化.这些基因分别属于生物大分子的合成代谢、三羧酸循环及呼吸代谢以及结瘤固氮过程等多个功能组,预示着根瘤中细菌的生长状态发生了显著的变化.在根瘤中,fixK以及受其激活的基因在nifA突变菌中的表达量显著高于野生菌.定量实时PCR分析表明,根瘤中fixLJ的转录水平在nifA突变菌中显著高于野生菌.通过统计学方法从13个表达发生显著变化的基因的上游调控序列中找到推测的NifA结合位点.
作者 田哲贤 邹华松 李健 张远涛 刘影 俞冠翘 朱家璧 Silvia Rüberg Anke Becker 王忆平
机构地区 北京大学生命科学学院 中国科学院上海生命科学学院上海植物生理及生态研究所 Lehrstuhl für Genetik
出处 《科学通报》 EI CAS CSCD 北大核心 2006年第15期1787-1793,共7页 Chinese Science Bulletin
基金 国家重点基础研究发展计划(批准号:2001CB108901和2001CB108902) 国家自然科学基金(批准号:30470940) 德国Bundesministerium für Bildung und Forschung(批准号:031U213D) Deutsche Forschungsge-meinschaft(批准号:BIZ 7)资助项目.
关键词 转录组 NIFA FixJ 根瘤细菌
分类号 S154.3 [农业科学—土壤学]
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参考文献41

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二级参考文献16

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共引文献3

同被引文献57

  • 1吴桐,朱家璧,俞冠翘,沈善炯.苜蓿根瘤菌多拷贝固氮基因启动子对根瘤发育的抑制[J].中国科学（B辑）,1994,24(10):1053-1059. 被引量：3
  • 2姚振华,田哲贤,戴小密,Anke Becker,李健,严海芹,肖琰,朱家璧,俞冠翘,Silvia Rüberg,王忆平,邹华松.异源nifA基因对苜蓿中华根瘤菌nifA突变体的互补分析[J].科学通报,2006,51(19):2258-2264. 被引量：4
  • 3Zi Ying Gong Zhi Shui He Jia Bi Zhu Guan Qiao Yu Hua Song Zou.Sinorhizobium meliloti nifA mutant induces different gene expression profile from wild type in Alfalfa nodules[J].Cell Research,2006,16(10):818-829. 被引量：3
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科学通报
2006年 第15期

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