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哺乳动物驱动蛋白的计算基因组学分析与鉴定 被引量：4

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摘要 提供了一种新颖的、基于比较基因组学方法的全长驱动蛋白预测方法(full-lengthkinesinpredictionprogram,FKPP),用于哺乳动物中驱动蛋白质组的鉴定与研究.之前的预测认为,哺乳动物共含94个驱动蛋白,而用FKPP从哺乳动物的基因组中总共鉴定出134个可能的驱动蛋白基因.基于数据库中存在的片段序列,用FKPP鉴定出25个可能的全长驱动蛋白.此外,用FKPP方法发现人的动点马达蛋白CENP-E应包含2701个氨基酸,而不是当初根据克隆预测的2663个氨基酸.通过对CENP-E的cDNA进行重测序,并同时采用特异性识别FKPP预测的CENP-E多肽抗体予以实验评估,结果表明,FKPP预测的CENP-E氨基酸序列是正确的.为此,本研究利用FKPP对哺乳动物的驱动蛋白进行了重新分类.鉴于目前公共数据库含有较多非全长序列的蛋白片段,FKPP可提供一个具有显著效率且准确新颖的全长序列预测手段,用于驱动蛋白以及其他蛋白家族的分子鉴定.
作者 薛宇 刘丹 符传孩 窦震 周庆 姚雪彪
机构地区 中国科学技术大学细胞动力学实验室及合肥微尺度物质科学国家实验室
出处 《科学通报》 EI CAS CSCD 北大核心 2006年第14期1654-1665,共12页 Chinese Science Bulletin
基金 国家自然科学基金(批准号:39925018,30270293和30121001) 中国科学院知识创新工程(批准号:KSCX2-2-01) 国家重点基础研究发展计划(批准号:2002CB713700) 国家高技术研究发展规划(批准号:2001AA215331) 教育部博士点基金(批准号:20020358051)资助项目.
关键词 驱动蛋白 比较基因组学 CENP-E 全长驱动蛋白预测方法(FKPP)
分类号 Q78 [生物学—分子生物学]
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参考文献26

  • 1Brendza R P,Serbus L R,Duffy J B,et al.A function for kinesin Ⅰ in the posterior transport of oskar mRNA and Staufen protein.Science,2000,289(5487):2120-2122
  • 2Cleveland D W,Mao Y,Sullivan K F.Centromeres and kinetochores:From epigenetics to mitotic checkpoint signaling.Cell,2003,112(4):407-421
  • 3Guzik B W,Goldstein L S.Microtubule-dependent transport in neurons:Steps towards an understanding of regulation,function and dysfunction.Curr Opin Cell Biol,2004,16:443-450
  • 4Hirokawa N,Takemura R.Molecular motors and mechanisms of directional transport in neurons.Nat Rev Neurosci,2005,6:201-214
  • 5McIntosh J R,Grishchuk E L,West R R.Chromosome-microtu-bule interactions during mitosis.Annu Rev Cell Dev Biosl,2002,18:193-219
  • 6Yen T J,Li G,Schaar B T,et al.CENP-E is a putative kinetochore motor that accumulates just before mitosis.Nature,1992,359:536-539
  • 7Abrieu A,Kahana J A,Wood K W,et al.CENP-E as an essential component of the mitotic checkpoint in vitro.Cell,2000,102:817-826
  • 8Hirokawa N,Takemura R.Kinesin superfamily proteins and their various functions and dynamics.Exp Cell Res,2004,301:50-59
  • 9Yao X,Abrieu A,Zheng Y,et al.CENP-E forms a link between attachment of spindle microtubules to kinetochores and the mitotic checkpoint.Nat Cell Biol,2000,2:484-491
  • 10Yao X,Anderson K L,Cleveland D W.The microtubule-dependent motor centromere-associated protein E (CENP-E) is an integral component of kinetochore corona fibers that link centromeres to spindle microtubules.J Cell Biol,1997,139:435-447

同被引文献23

  • 1BIAN MingLei1, FU JingYan1, YAN Yan1, CHEN Qiang1, YANG Chao2, SHI QingHua2,JIANG Qing1 & ZHANG ChuanMao1 1MOE Key Laboratory of Cell Proliferation and Differentiation and the State Key Laboratory of Bio-membrane and Membrane Bio-engineering, College of Life Sciences, Peking University, Beijing 100871, China,2Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230026, China.Short exposure to paclitaxel induces multipolar spindle formation and aneuploidy through promotion of acentrosomal pole assembly[J].Science China(Life Sciences),2010,53(11):1322-1329. 被引量：3
  • 2Ohira Y.Effects of denervation and deaf rentation on mass and enzyme activity in rat skeletal muscles[J].Jpn I Physiol,l999,39(1):21-31.
  • 3Hawke TJ,Garry DJ.Myogenic satellite cells:physiology to molecular biology[J].J Appl Physiol,2001,91(2):534-551.
  • 4Yoshida N,Yoshida S,Koishi K,et al.Cell heterogeneity upon myogenic differentiation:down-regulation of MyoD and Myf-5 generates 'reserve cells'[J].J Cell Sci,1998,111(Pt6):769-779.
  • 5Stuelsatz P,Pouzoulet F,Lamarre Y,et al.Down-regulation of MyoD by calpain 3 promotes generation of reserve cells in C2C12 myoblasts[J].J Biol Chem,2010,285(17):12670-12683.
  • 6Vale RD,Milligan RA.The way things move:looking under the hood of molecular motor proteins[J].Science,2000,288(5463):88-95.
  • 7Sawin KE,LeGuellec K,Philippe M,et al.Mitotic spindle organization by a plus-end-directed microtubule motor[J].Nature,1992,359(6395):540-543.
  • 8Neef R,Preisinger C,Sutcliffe J,et al.Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis[J].J Cell Biol,2003;162(5):863-876.
  • 9Le Guellec R,Paris J,Couturier A,et al.Cloning by differential screening of a Xenopus cDNA that encodes a kinesin-related protein[J].Mol Cell Biol,1991,11(6):3395-3398.
  • 10Valensin S,Ghiron C,Lamanna C,et al.KIF11 inhibition for glioblastoma treatment:reason to hope or a struggle with the brain[J].BMC Cancer,2009(9):196-200.

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科学通报
2006年 第14期

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