外植体与不同理化因子对虎杖愈伤组织诱导及次生代谢产物形成的影响(英文)

Effects of Explants,Medium Formulations and Light on Callus Induction and Secondary Metabolites Accumulated in the Calli of Polygonum cuspidatum (Polygonaceae)

考察了外植体、培养基及光照条件对中药植物虎杖愈伤组织形成及次生代谢产物生产的影响。总的来看，所有愈伤组织中总酚和总黄酮含量比原植物的含量高2—3倍；而蒽醌的含量比原植物中含量低。外植体对愈伤组织形成及次生代谢产物生产的影响很大，所考察的3种外植体中。叶外植的愈伤组织诱导率最高而源于根外植体的愈伤组织具有最好的次生代谢能力。所考察的6种培养基中，MS＋0．5mg／L 2，4-D＋1．0mg／L 6-BA和N6＋0．5mg／L 2，4-D＋1．0mg／L 6-BA无论对于愈伤组织的产生还是次生代谢产物的累积都有较优表现。光照对愈伤组织诱导及次生代谢产物产生有明显影响。但二者无规律性联系。

Callus initiation of a Chinese traditional medical plant, Polygonum cuspidatum Sieb. et Zucc （Polygonaceae） was studied, with a focus on the factors that could influence the callus induction ability and secondary metabolites accumulation. In comparison with the parent explants, the levels of total phenolics and flavones were 2 to 3-fold higher and the levels of anthraquinones （AQs） were lower in all ealli induced. Different explants exhibited great variability in callus induction rate and secondary metabolite accumulation. Among the three explants tested, leaf explants were with the best capacity in callus induction, while root explants accumulated the highest levels in secondary metabolites. Two media of six tested, MS and N6 respectively plus 0.5 mg/L 2, 4-dichlorophenoxy acetic acid and 1.0 mg/L 6-benzylaminopurine, showed the best performances in both callus growth and secondary metabolite accumulation. Illumination shower a remarkable effects on both callus induction and secondary metabolite accumulation, but no regular pattern was observed.