摘要
AIM： To detect microsatellite instability （MSI） in patients with hereditary nonpolyposis colorectal cancer or ordinary hereditary colorectal cancer and to provide criteria for screening the kindreds with hereditary nonpolyposis colorectal cancer at molecular level.METHODS： MSI was detected in the specimens from 20 cases with HNPCC, 20 cases with ordinary hereditary colorectal cancer and 20 cases with sporadic colorectal cancer by means of polymerase chain reaction-single strand conformation polymorphism. RESULTS： The positive rate of MSI was 85% （17/20） in HNPCC group, 40% （8/20） in ordinary hereditary colorectal cancer group and 10% （2/20） in the sporadic colorectal cancer group respectively. The differences were significant. The mean ages of the three groups were 43.6, 52.2, and 61.8 years respectively, which increased gradually. The incidence of right hemicolon cancer was 64.7%, 37.5%, and 0% respectively, which decreased gradually and had significant difference. The expression ratio of BAT26 and BAT25 was 94.1% respectively, which was highest in the 5 gene sites studied. The incidence of poorly differentiated adenocarcinoma was 70.6% in HNPCC group among high frequency microsatellite instability （MSI-H）, which was higher than the other two groups, which had 50% and 50% respectively. CONCLUSION： The incidence of MSI-H is higher in HNPCC group. The detection of MSI is simple and economical and has high correlation with the clinicopathologic feature of HNPCC and can be used as a screening method to detect the germ line mutation of the mismatch repair gene.
瞄准：与世袭 nonpolyposis 颜色在病人检测微卫星不稳定性(MSI ) 表面的癌症或平常的世袭颜色表面的癌症并且为与世袭 nonpolyposis 颜色屏蔽家族提供标准在分子的水平的表面的癌症。方法：MSI 与 HNPCC 从 20 个盒子在标本被检测，有平常的世袭颜色的 20 个盒子有分散的颜色的表面的癌症和 20 个盒子借助于聚合酶链反应单人赛的表面的癌症搁浅符合构造多型性。结果：MSI 的积极的率是 85%(17/20 ) 在 HNPCC 组织， 40%(8/20 ) 在平常的世袭颜色，表面的癌症组织并且 10%(2/20 ) 在分散的颜色，表面的癌症分别地组织。差别是重要的。三个组的吝啬的年龄分别地是 43.6， 52.2，和 61.8 年，它逐渐地增加了。正确的半结肠癌的发生分别地是 64.7% ， 37.5% ，和 0% ，它逐渐地减少了并且有有效差量。BAT26 和 BAT25 的表示比率分别地是 94.1% ，它在地点学习了的 5 基因是最高的。糟糕区分的腺癌的发生在在高频率之中的 HNPCC 组是 70.6% 分别地有 50% 和 50% 的微卫星不稳定性(MSI-H ) 比另外的二个组高，。结论：MSI-H 的发生在 HNPCC 组是更高的。MSI 的察觉简单、节俭并且与 HNPCC 和罐头的临床病理特征有高关联被用作一个屏蔽方法检测失配修理基因的细菌线变化。
