SARS期间住院儿童SARS-CoV(IgG)抗体水平回顾性分析

A retrospective analysis of severe acute respiratory syndrome-CoV(IgG) antibody in hospitalized children during the epidemic

目的回顾性分析SARS期间在院儿童SARS-CoV(IgG)抗体的出现规律,探讨儿童与成人产生SARS-CoV(IgG)抗体的差异。方法ELISA及蛋白芯片法检测85例在院儿童SARS-CoV(IgG)抗体水平。结果在院患者中儿童比成人抗体阳性率要高(17·6%vs6·3%,P<0·05),其中白血病患儿阳性率最高,达31·6%,蛋白芯片技术分析发现主要为病毒N、3CL、S1蛋白抗原反应阳性。结论在院儿童具有较高的SARS-CoV(IgG)抗体阳性率。

Objective To retrospectively analyze the expression of SARS-CoV （IgG） in the serum of hospitalized children who were not suffering from SARS, and retrospectively evaluate the difference of generation of the antibody between the children and adults. Methods Serum samples were collected from 85 hospitalized children, 500 health-check adults, 976 hospitalized adults, and 156 adults recovered from severe acute respiratory syndrome （SARS）, and SARS-CoV （IgG） in the specimens was determined by ELISA and biologic chip. Results The level of serum SARS-CoV （IgG） in childhood patients was significantly higher than that in adult patients （17. 6% vs 6. 3%, P〈0. 05）, but it was much lower than that in adults who recovered from severe acute respiratory syndrome （17. 6% vs 75. 6%, P〈0. 01）. The SARS-CoV （IgG） in serum of hospitalized children could react with N, 3Cl and Sl antigen proteins of SARS-CoV. The antibodies in serum of adults recovered from SARS, however, could react with all 7 antigen proteins including N, M, 3CL, S1, S2, S3 and S4 of SARS-CoV. Conclusion There is higher level of SARS-CoV （IgG） in hospitalized children than that in adult patients.