恒定磁场对骨组织中细胞影响的研究

Effect of permanent-magnetic field on cells in bone

目的阐明恒定磁场对成骨细胞和破骨细胞的生长和功能的影响和对骨髓基质细胞向成骨细胞方向分化的作用,从而在细胞水平较全面的解释磁场治疗骨质疏松的机理。方法利用体外原代培养的成骨细胞、破骨细胞和骨髓基质细胞,分别外加0.38T、0.48T恒定磁场处理后,观察恒定磁场对这三种细胞生长、分化和功能的影响。结果恒定磁场处理可促进骨髓基质细胞向成骨细胞方向分化,促进成骨细胞的增殖、分化及功能的表达,并且抑制破骨细胞的生长、分化和功能。结论恒定磁场促进骨组织的成骨作用,抑制骨分解,是其治疗骨质疏松良好效果的部分机制。

Objective To investigate the effect of permanent-magnetic field on the growth and function in osteoblast and osteoclast and its effect on bone marrow stromal cells differentiation into osteoblast in order to clarify the therapeutic mechanism of osteoporosis. Methods After exposure to 0.38 T and 0.48 T permanent-magnetic field on primary in vitro cultured osteoblast, osteoclast and bone marrow stromal cells, the effects of growth, differentiation and functions were observed. Results The differentiation of bone marrow stromal cells into osteoblast was promoted by the permanent-magnetic field exposure, and the proliferation, differentiation and function of the osteoblast was also improved, but the growth, differentiation and function of the osteoclast was suppressed. Conclusions Permanent-magnetic field could accelerate bone formation and inhibit bone absorption, thus being the partial mechanism for osteoporosis management.