组织芯片在免疫组化染色阳性对照中的应用

Tissue microarrays:a new positive control of immunohistochemical staining

目的应用组织芯片制作一种高效方便的免疫组化阳性对照片。方法选择60例不同病变不同抗体染色的免疫组化阳性片和相应的组织蜡块,使用组织芯片制备仪制成组织芯片蜡块,经过连续切片,共获得150张组织芯片,每张组织芯片包含77个组织芯点位,每个组织点的直径为0·6mm,芯片中组织所占面积为0·8cm×0·5cm。每张芯片至少包含了50种常见的抗原。然后将常规工作中测试片裱贴在同一张芯片组织的旁边,同时进行免疫组化染色。结果常见的数10种抗体染色,总能在芯片中出现至少1个阳性点位,其余点位则成为了阴性对照。旁边的测试组织是否阳性有了明确的对照。结论与传统的阳性对照片相比,组织芯片阳性对照片具有更高的效率,参考值更多,更科学,并且简便易行。

Purpose To develop an efficient and convenient positive control of immunohistochemical staining. Methods 60 cases of different positive sections of immunohistochemical staining and their paraffin blocks were chosen. A tissue microarrays block with 0. 6 mm in diameter tissue core was constructed by using the tissue microarrayer. After continuous cutting, 150 sections were obtained. The microarray contained 77 tissue cores, with a dimension of 0.8 cm×0.5 cm and a wide antigen profile. One section of tissue microarray was as a positive control on each slide of the patient tissue to undergo identical immunohistochemical procedures. Results During the immunohistochemical procedures, at least one of microarray cores presented positive and the others were as the negative control. Then, we had a definite contrast to interpret whether the tested tissue was positive or not. Conclusion Compared with conventional positive control, the microarray positive control is more efficient, scientific, simple and applicable.