几种中药提取物和药物对乙醇脱氢酶活性影响的研究

Studies on the Effect of Extracts of Several Chinese Herbal Medicines and Other Medicines on Alcohol Dehydrogenase Activity

目的:探讨了六种中药水提取物和醇提取物及几种药物对乙醇脱氢酶活性的影响,为中药解酒保肝药物的开发提供酶学依据。方法:选择古方中常用的几味解酒保肝中药及临床常用的药物,应用瓦勒-霍赫(Valle&Hoch)法体外测定这些药物对乙醇脱氢酶活性的影响。结果:白豆蔻和葛花的水提物、葛花醇提物、市售解酒保肝茶、九宝、葛花王、L-半胱氨酸,三七皂甙等对乙醇脱氢酶有激活作用,激活率分别为2.8%、16.0%、3.3%、40.1%、16.0%、14.3%、30.5%、10.1%。而葛根、三七、甘草、枳椇子、芦荟的水提物和醇提物、白豆蔻醇提物、盐酸雷尼替丁对乙醇脱氢酶的活性有抑制作用。结论:其中,有些药物可以通过提高乙醇脱氢酶活性来降低乙醇浓度,这可能是解酒药的作用机制之一。而其它药物的解酒机制不能单纯从酶学方面解释。

Objective ： To study the effects of water and alcohol extracts of several Chinese herbal medicines and other medicines on alcohol dehydrogenase activity in order to provide enzymology basis on new medicine. Methods ： Water or alcohol extracts of Chinese herbal medicine and other medicine were tested on the effects of alcohol dehydrogenase activity by Valle ＆ Hoch method. Results： A- mong them, 8 were found to have the effect of activation on alcohol dehydrogenase. They were water extracts of Amomum kravanh and Pueraria flowers, the alcohol extracts of Pueraria flowers, compound hepatcare Chinese medicine and compound Pueraria medicine, Lcysteine, notoginseng saponin. Others had inhibiting action. Conclusion： To decrease alcohol concentration in the body through activating the activity of ADH may be one of the mechanisms for some traditional Chinese herbal medicine in neutralizing the effect of alcohol drink.