水环境中RH-5992液相色谱分析

Determination of RH-5992 in Aquatic Environment by Liquid Chromatography.

为评价RH-5992在环境中的分布与结局,介绍了一种该化合物在湖水、淤泥、鱼、宽叶香蒲草和驴蹄草中的残留量分析方法.分析过程包括溶剂的提取、液液分配、柱层析净化和液相色谱分析.分析物采用二极管阵列式(diode-array)紫外检测器进行检测,柱型为RP-8,流动相为乙腈、二氧六环和水.方法平均回收率为85%—98%,变异系数为6%—9%,最低检出浓度除湖水为0.050 μg/L外,其它几种样品为0.009—0.028 mg/kg。

A method was developed to determine RH-5992 in some aquatic environment matrixes. The procedure included solvent extration, liquid-liquid partition, columm cleanup and liquid chromatographic determination. RH-5992 was analyzed on a liquid chromatograph equipped with a diode-array UV detector set at 236 nm, using a RP-8, 10 μm column with a mobile phase of acetonitrile-dioxane-water. Mean recoveries for analyte ranged from 85%to 98%, with coefficient of variation from 6% to 9%. Limits of detection were 0. 050 mg/kg for natural waters and from 0. 009 to 0. 028 mg/kg for other matrixes.