经辐照氟银处理脐静脉的抑菌效果观察

BACTERIOSTATIC EFFECT OF IRRADIATED HUMAN UMBILICAL VENOUS INCORPORATED WITH NOFLOXACIN AND SILVER

目的观察经辐照氟银处理脐静脉的抑菌效果。方法脐静脉随机分为3组:冷冻脐静脉组(Ⅰ组)、冻干辐照脐静脉组(Ⅱ组)、辐照氟银脐静脉组(Ⅲ组);对辐照氟银组脐静脉进行含银量测量,细菌培养及抑菌实验并与其他组进行比较。结果细菌培养各组均无细菌生长;辐照氟银脐静脉组含银(376.5±23.0)μg/g;具有抑菌作用,与其他各组相比,差别显著(P<0.01)。结论辐照氟银脐静脉具有抗菌作用,可用来预防移植后感染导致的手术失败。

Objective To abserve the bacteriostatic effect of irradiated human umbilical venous incorporated with nofloxacin and silver （IHUVINS）. Methods Human umbilical venouses were randomly divided into three groups： deepen-frozen human umbilical venouses group （group Ⅰ ）, lyophilize-irradiated human umbilical venouses group（group Ⅱ ）, and irradiated human umbilical venous incorporated with nofloxacin and silver group（group Ⅲ）. They are assayed with bacteri- um culture and inhibiting bacteria in vitro, and Ag^＋ level was detected in the IHUVINS. Result Ag＋ level was （376.5 ± 23.0） μg/g in the IHUVINS, and no bacterial growth was found in group Ⅰ-Ⅲ. By contrast, the IHUVINS effectively inhibited the growth of bacteria（P〈0.01）. Conclusion Irradiated human umbilical venous incorporated with nofloxacin and silver has good bacteriostatic effect, and can avoid operational failures caused by infection and cross-infection after transplantation.