血管内皮细胞生长因子检测在重型肝炎患者中的应用

Appliciton of testing VEGF in patients of severe hepatitis

目的:探讨检测血管内皮细胞生长因子(VEGF)水平在重型肝炎患者中的临床应用,为其预后提供早期诊断依据。方法:采用双抗体夹心法定量检测80例重型肝炎患者血清VEGF水平。结果:急、慢性重型肝炎患者VEGF检出率分别为39·09%和81·36%。慢性期恢复率升高差异有显著性意义(P<0·01),存活组和死亡组检出率分别高于正常组,存活组恢复期检出率达81·96%(P<0·01),而死亡组恢复期检出率明显下降达21·05%(P<0·05)。血清VEGF检测灵敏度为15pg/ml,特异性强,重复性好,板内板间变异系数均<9·5%。结论:血清VEGF水平检测具有较好的特异性和敏感性,可反映重型肝炎患者肝细胞再生程度,是临床观察重型肝炎患者预后具有特征性的疗效指标。

Objective： To discuss the clinical application of VEGF level in severe hepatitis patients and hence provide the early diagnositc criteria for the patients＇ prognosis. Methods： VEGF serum level in 80 cases of severe hepatitis was quantitively detected by using Two - antibody - sandwich ELISA.. Results： The positive rate of severe hepatitis cases is 39.09% in acute stage and 81.36% in chronic stage. Increases of recovery rate in chronic stage have significant difference （P 〈0. 01 ） ; but the positive rate for dead group in recovery stage decrease by 21, 05% （ P 〈 0. 01 ） The testing acuity for serum VEGF is 15 pg/ml, and it has good specificity and repeatability. The coefficient of variance in intraplates and interplates is less than 9. 5%. Conclusion. The VEGF level which can reflect the regeneration degree of hepatic cells in severe hepatitis cases and owns good specificity and sensitivity is a specific index for observing the prognosis of severe hepatitis patients in clinic.