电焊作业对工人血清去甲肾上腺素含量和乙酰胆碱酯酶活性的影响

Effect of welding operation on noradrenalin level and acetylcholinesterase activity in serum of workers

以某金属结构厂65名电焊作业工人为接触组,另以不接触毒物的49名非电焊工人为对照组,采用酶联免疫吸附法(ELISA)测定血清去甲肾上腺素(NE)含量,用比色法测定血清乙酰胆碱酯酶(AChE)活性。同时检测受试者血锰浓度。结果接触组NE含量为(266.69±211.63)pg/ml,明显高于对照组〔(164.68±130.45)pg/ml〕(P<0.01);接触组AChE活性为(72.35±13.61)U/ml,明显高于对照组〔(60.50±13.00)U/ml〕(P<0.01),接触组血锰为(0.778±0.389)μmol/L,明显高于对照组〔(0.546±0.321)μmol/L〕(P<0.01)。提示电焊作业可导致工人血锰浓度增加,同时引起血清NE含量和AChE活性升高。

Sixty-five welders and 49 non-welding workers were selected as exposed group and control group, respectively. The serum noradrenalin （NE） level was determined by ELISA method, the serum acetylcholinesterase （ACHE） activity was determined by spectrometric method. Additionally, the blood manganese content, air manganese levels in working places were also determined by graphite stove atomic absorption method. The result showed that the serum NE level [ （266.69 ± 211.63） pg/ml] and ACHE activity [ （72.35± 13.61） U/ml] in welding workers were significantly higher than that of the controls [ （164.68 ± 130.45） pg/ml] and [ （60.50 ± 13.00） U/ml] respectively （ P 〈 0.01）. The blood manganese content in welding workers [ （0.778 ± 0.389） μmol/L] was also significantly higher than that in the control [ （0.546 ± 0.321） μmol/L] （ P 〈 0.01 ）. It is suggested that welding work may induce blood manganese content rising, sermn NE level increasing and ACHE activity enhancing.