外源激素对丹参愈伤组织诱导和有效成分含量的影响

A Study on the Effect of Different Exogenous Hormones on Callus Induction and Bioactive Constituent of Salvia miltiorrhiza

以丹参(S a lv ia m iltiorrh iza)无菌苗的叶为外植体,接种于附加2,4-D、NAA、6-BA及其组合的M S固体培养基上,研究了外源激素对愈伤组织的诱导及有效成分含量的影响。结果表明:单独使用3种外源激素在一定浓度范围内均能诱导外植体产生愈伤组织;2,4-D对丹参愈伤组织的生长有促进作用,但当2,4-D的浓度在4 m g/L以上时,对愈伤组织的生长有抑制作用;配合使用外源生长素和细胞分裂素,能获得出愈率高、生长快、质量好的愈伤组织;外源激素的适宜组合为2,4-D 2.0 m g/L+6-BA 1.0 m g/L;愈伤组织中丹酚酸B的含量随培养时间的延长呈下降趋势。

Salvia miltiorrhiza aseptic leaves were used as explants cultured in solid medium MS supplemented with 2,4-D, NAA, 6-BA or combinations of plant exogenous hormones with different concentrations, to study their the effect on callus induction and the bioactive component accumulation. The results showed that all the three exogenous hormones could induce callus under certain concentration ranges. Better results could be obtained by combining three exogenous hormones, the optimum combination was Ms＋ 2,4-D2. 0 mg/L＋6-BA1. 0 mg/L. Higher concentration of 2,4-D （over 4 mg/L） could restrain callus growth. The content of salvianolic acid B reduced with the increase of induction time.