外源水杨酸对水稻(Oryza sativa L.)幼苗根的NaCl胁迫缓解效应

Relieving Effect of Exogenous Salicylic Acid on Rice (Oryza sativa L.) Seedling Roots under NaCl Stress

为研究25 mg/L外源水杨酸对0,50,100 mmol/L NaCl处理下水稻幼苗根的胁迫缓解效应,测定了根组织内脯氨酸含量、可溶性糖含量、氨同化关键酶活性等相关指标.结果表明外源水杨酸可使组织内脯氨酸含量显著降低至与正常对照相当水平,而可溶性糖含量升高,说明外源水杨酸存在时可溶性糖作为主要渗透胁迫调节物并对盐胁迫适当缓解,表现在幼苗根谷氨酰胺合成酶及依赖于NADH的谷氨酸脱氢酶的活性上升20%左右、可溶性蛋白质含量的提高,氮同化加强而对盐胁迫有一定的抗性.对50 mmol/L NaCl胁迫处理的材料缓解效应较显著.

To study the effect of 25 mg/L salicylic acid（SA） on rice（Oryza sativa L. ） seedling roots under NaCI（0,50,100 mmol/L）, content of proline, soluble sugar and activity ofammonium assimilation enzymes of rice root and relative parameter were detected. 25 mg/L SA decreased roots proline content and increased total soluble sugar content dramatically under NaCl stresss. And plant adapted salinity when soluble sugar as main compatible osmolyte in following aspacts. Activities of the key enzymes of nitrogen assimilation, such as glutaminesynthetase （GS） and NADH dependent glutamate dehydrogenase （NADH-GDH） was induced, and soluble protein content increased, that is to say nitrogen assimilation was enhanced.