HPLC法测定果实中花色素苷含量

Determination of anthocyanin content in fruits by HPLC

建立了果实中花色素苷的HPLC测定方法，采用ODS-C18柱（250nm×4．6nm），流动相A：1．6％甲酸水溶液；B：1．6％甲酸甲醇溶液，梯度洗脱为0～5min，85％A＋15％B；5～10min，80％A＋20％B；10～30min，72％A＋28％B；30-36min，40％A＋60％B；36-48min，100％B。检测波长510nm，流速为1mL／min。2种花色素苷在0．00104～0．0104mg／mL范围内线性良好，最低检出限为0．02μg/mL，平均回收率为99．5％和99．25％，平均标准偏差为1．98％和2．50％，并测定了大石早生李和藤稔葡萄中花色素苷含量。

The method of determining anthocyanin content in fruits by HPLC was established. A ODS- C18 column （250 nm × 4.6 nm） was selected. The mobile phase A and B was water and methanol containing 1.6% formic acid respectively. The gradient elution was 0--5 min, 85%A＋ 15%B, 5--10 min, 80%A＋ 20%B, 10--30 min, 72%A＋28%B, 30--36 min, 40%A＋60%B, and 36--48 min, 100%B. The detection wavelength was 510 nm, and the flow rate 1 mL/min. The calibration curves for cyanin-3-glucoside and cyanin-3-rutiniside were linear within the range of 0. 00104-0. 0104 mg/mL. The mean recovery ratios for them were 99.5 % and 99.25 %, and the mean standard deviations for them 1.98 % and 2.5 %. The detection limit of this method was 0.02μg/mL. The method was applied to Dashizaosheng plum and Fujiminori grape samples.