O型口蹄疫病毒VP1基因的克隆及原核表达载体构建

Cloning and Construction of Prokaryotic Expression Vector of VP1 Gene of Foot-and-mouth disease virus Serotype O

用Trizol提取O型口蹄疫病毒RNA,根据已经公布的O型口蹄疫病毒核苷酸序列,设计合成1对VP1基因的引物,通过RT-PCR扩增出VP1基因,将其克隆至表达载体pET-32a中。经测序表明,目的基因VP1已正确地整合至表达质粒中。

The RNA of FMDV serotype O was abstracted by Trizol. According to the published nucleotide sequence of the gene of foot-and-mouth disease virus serotype O, a pair of primers were designed and synthesized to clone the VP1 gene. The gene of FMDV was amplified by RT-PCR and subsequently inserted into the expression vector pET-32a. The VP1 gene was sequenced and compared with other published FMDV type O strains in the GeneBank. The VP1 gene was successfully conformed into the expression vector.