摘要
In Antarctic, the geography and climate differs from those in other places, and the baeteria there have adapted well to the environment there. Two hundred strains of bacteria were isolated from the sea ice in Antarctica. The bacteria were sereened for DHA by means of GC, with fish oil as the standard. Seven strains eontaining DHA or EPA were obtained, among whieh the strain of No. N-6 was outstanding. And the eomponent of DHA was identified by CC-MS. The relative eontent of DHA in N-6 was 8.72%, and total lipids in dry bacteria was 22.54%. The effects of some faetors, ineluding temperature, salinity and pH value, on the growth and DHA-content of strain N-6 were studied. The results show that the DHA-content is relatively high when in low temperature and high pH, and the bacterium is psyehrophilic, alkalophilie.
In Antarctic, the geography and climate differs from those in other places, and the baeteria there have adapted well to the environment there. Two hundred strains of bacteria were isolated from the sea ice in Antarctica. The bacteria were sereened for DHA by means of GC, with fish oil as the standard. Seven strains eontaining DHA or EPA were obtained, among whieh the strain of No. N-6 was outstanding. And the eomponent of DHA was identified by CC-MS. The relative eontent of DHA in N-6 was 8.72%, and total lipids in dry bacteria was 22.54%. The effects of some faetors, ineluding temperature, salinity and pH value, on the growth and DHA-content of strain N-6 were studied. The results show that the DHA-content is relatively high when in low temperature and high pH, and the bacterium is psyehrophilic, alkalophilie.
