六味地黄汤对大鼠单侧输尿管结扎后肾小管间质ColIV及TIMP-1表达的影响

Effect of Liuweidihuang Decoction on Expression of Tubulointerstitial TIMP-1 and ColIV in Unilateral Ureter Ligation Rats

目的观察六味地黄汤对单侧输尿管梗阻(UUO)大鼠模型中胶原IV(ColIV)及基质金属蛋白酶抑制物-1(TIMP-1)表达的影响。方法将输尿管梗阻大鼠随机分为模型组,依那普利组,六味地黄汤组,假手术组,14d后采用免疫组织化学检测ColIV和TIMP-1的表达水平。结果与假手术组比较,其余3组ColIV和TIMP-1表达均明显高于假手术组(P<0.01),六味地黄汤组ColIV和TIMP-1表达明显低于模型组(P<0.01),高于依那普利组(P<0.05)。结论六味地黄汤可下调TIMP-1和ColIV的表达,减缓UUO大鼠肾小管间质损害,可作为梗阻性肾病辅助用药。

Objective To observe the effect of Liuweidihuang decoction（LD） on expression of met＇,dloproteinase-1（TIMP-1） and collagen type Ⅳ （ColⅣ） in unilateral ureter obstruction（UUO） rats, Methods UUO rats were divided into four groups： sham surgery group（J）, model group （M）, Enalapril group（Y） and LD group（Z）. After 14 days the expression of TIMP-1 and ColⅣ was detected by immunohistochemistry. Resluts Compared with the sham surgery group, the level of TIMP-1 and ColⅣ expression was obviously higher than other three groups （P〈0.01） were. LD group was lower than that in model group（P〈0.01）, the TIMP-1 and ColⅣ of in LD group was higher than that in Enalapril group（P〈0.05）. Conclusion LD can decrease the expession of UUO TIMP-1 and ColⅣ level and reduce tubulointerstitial injury, which shows that LD can be an adjuvant for obstructive kidney disease.