摘要
目的观察六味地黄汤对单侧输尿管梗阻(UUO)大鼠模型中胶原IV(ColIV)及基质金属蛋白酶抑制物-1(TIMP-1)表达的影响。方法将输尿管梗阻大鼠随机分为模型组,依那普利组,六味地黄汤组,假手术组,14d后采用免疫组织化学检测ColIV和TIMP-1的表达水平。结果与假手术组比较,其余3组ColIV和TIMP-1表达均明显高于假手术组(P<0.01),六味地黄汤组ColIV和TIMP-1表达明显低于模型组(P<0.01),高于依那普利组(P<0.05)。结论六味地黄汤可下调TIMP-1和ColIV的表达,减缓UUO大鼠肾小管间质损害,可作为梗阻性肾病辅助用药。
Objective To observe the effect of Liuweidihuang decoction(LD) on expression of met',dloproteinase-1(TIMP-1) and collagen type Ⅳ (ColⅣ) in unilateral ureter obstruction(UUO) rats, Methods UUO rats were divided into four groups: sham surgery group(J), model group (M), Enalapril group(Y) and LD group(Z). After 14 days the expression of TIMP-1 and ColⅣ was detected by immunohistochemistry. Resluts Compared with the sham surgery group, the level of TIMP-1 and ColⅣ expression was obviously higher than other three groups (P〈0.01) were. LD group was lower than that in model group(P〈0.01), the TIMP-1 and ColⅣ of in LD group was higher than that in Enalapril group(P〈0.05). Conclusion LD can decrease the expession of UUO TIMP-1 and ColⅣ level and reduce tubulointerstitial injury, which shows that LD can be an adjuvant for obstructive kidney disease.
出处
《湖南中医学院学报》
2006年第4期22-24,共3页
Journal of Hunan College of Traditional Chinese Medicine
基金
湖南省教育厅课题(03C305)