摘要
应用放射配体结合测定法，研究老年鼠睾丸糖皮质激素受体（ＧＲ）的变化。结果表明，老年鼠睾丸细胞液ＧＲ的最大结合容量（Ｒｏ）为４８．４３±１１．８７ｆｍｏｌ／ｍｇ蛋白，显著低于成年鼠的５９．６９±１２．７３ｆｍｏｌ／ｍｇ蛋白（Ｐ＜０．０１），ＧＲ的平衡解离常数（Ｋｄ）无显著性变化（Ｐ＞０．０５）。同时测定了血浆皮质醇、睾酮浓度及外周血白细胞ＧＲ的特异结合量（Ｒｓ）和睾丸细胞液磷脂酶Ａ２的含量，探讨了ＧＲ变化与衰老发生的关系。
The glucocorticoid receptors(GR) of testicular cytosol in the aged rats were estimated by radioligand binding method. It was found that the numbers of [3H]-Dex maximal binding capacity(Ro) in aged rats were significantly lower than that of the adult rats(48.43±11.87 vs 59.69±12.73fmol/mg·protein,P<0.01),dissociation constant(Kd) of GR did not change (P>0.05).The concentration of plasma cortisol, testosterone and the numbers of [3H]-Dex specific binding capacity(Rs) in peripheral leukocytes and phospholipase A2 of testicular cytosol were ditermined simultaneously. The results indicated that a decrease in numbers of GR is probably related to the ageing.
