摘要
2001—2005年对蚕豆组织培养快繁技术体系的建立进行了整套、深入细致的研究,研究结果表明:蚕豆茎节是适宜快繁的外植体;芽增殖最适培养基(单位:mg/L,下同):N6+NAA0.1—1.0+BA0.5—4.0+肌醇100—300;壮苗培养基是:N6+NAA0—0.5+BA0—0.5+多效唑1.0—3.0;生根培养基是:N6+NAA0.4—0.5。
Taking the stem segmnts with lateral buds as explants,the tissue culture of faba bean were studied from 2001 to 2005. The results showed that the stem of faba bean is suitable explants for its rapid propagation;N6 ＋ NAA0. 1-1.0 ＋ BA0.5-4.0 ＋ Inositol 100-300 gave the best result for subculture of shoots and the multiplication rate; N6 ＋ NAA0-0.5 ＋ BA0-0. 5 ＋ ppp1.0-3.0 for robust seedling; N6 ＋ NAA0.4-0.5 for exvitro shoots rooting.
出处
《青海农林科技》
2006年第3期1-3,6,共4页
Science and Technology of Qinghai Agriculture and Forestry
关键词
春蚕豆
组织培养
快繁
研究
Faba bean
Tissue Culture
Rapid propagation
Study
