摘要
为了探讨Alzheimer病患者海马神经元退变与钙-钙调素的关系,以尸检人脑海马组织为研究对象,根据患者年龄分为青年组、老年组和Alzheimer病组(前两组为对照组)。在钙vonKossa法染色后,借助显微分光光度计观察海马神经元中钙离子的变化,在荧光染色后通过显微荧光光度计了解钙调素的相对活性。结果显示与对照组比较,钙离子在老年组和Alzheimer病组中增高,尤其是在Alzheimer病组;钙调素相对活性则明显增加,且有统计学意义。老年人和Alzheimer病患者钙沉积增加,提示体内钙平衡有一定变化,钙调素相对活性增加,提示神经元退变的过程可能同时伴随钙相对调节功能的增加,钙的内环境稳定和钙调素的相对活性的变化在某种程度上,可能对Alzheimer病神经元退变起一定的作用。
Theauthorsobservedtherelationshipbetweenthecalcium-calmodulinchangesandtheneuronaldegenerationinthehippocampusofpatientswithAlzheimer′sdisease.Thehumanhip-pocmpaltissuespostmotemweredividedintothreegroups,namely,theyoung,theagedandAlzheimer′sdiseasegroups.Somehippocampaltissueswerestainedbymeansofvonkossa′sstainothersdyedwithfluorescence.Thecalciuminthehippocampalneuronswasobservedwiththehelpofmicrospectrophotometer.Thecalmodulinactivityinthehippocampalneuronswasinvestigatedwiththehelpofamicrospectrofluorometer.Theresultsdisclosedthatthedensitometricmeasure-mentoftheintensityofcalciumintheneuronsshowedanincreaseintheaged,especialyinAlzheimer′sdiseasegroupascomparedwiththecontrols.Thedensitometricmeasurementofthein-tensityofcalmodulinactivityintheneuronsrevealed′ssignificantincreaseinAlzheimer′sdiseasegroupsconparedwiththecontrols.TheauthorsconsideredthatthedepositionofcalciummightshowachangeofthecalciumhomeostasisduringtheperiodofagingandAlzheimer′sdisease.Increaseincalmodulinactivetymaydemonstratedthatincreasedcalciumrelatedfunctioncouldcoexistwiththedegenerativeprocessinsomeneurons.Insummary,itwasconsideredthatthechangesofthecalciumhomeostasisandthecalmodulinactivitymightplayaroleinawayinthehippocampalneuronaldegen-eratingprocessinAlzheimer′sdisease.Furtherstudywouldbeneededformoreexplorationandclari-fication.
出处
《中华神经科杂志》
CAS
CSCD
1996年第5期289-292,共4页
Chinese Journal of Neurology
基金
全军"八五"医药卫生科研基金
关键词
痴呆
老年性
海马
钙
调钙蛋白
Alzheimer′sdiseaseHippocampusneuronCalciumCalmodulin