食管鳞癌组织中血管内皮生长因子mRNA的表达

Expression of the vascular endothelial growth factor in esophageal cancer tissue

目的:探讨食管癌组织中血管内皮生长因子(VEGF)的表达水平与肿瘤侵袭和转移的关系。方法:应用实时荧光定量RT-PCR及基因芯片技术对15例食管鳞癌组织和相应正常食管黏膜组织进行VEGFmRNA检测。结果:15例食管鳞癌组织中有8例VEGFmRNA上调表达,其平均表达水平,cDNA微阵列和实时荧光定量RT-PCR检测结果分别为(3.82±0.75)和(4.11±0.89);VEGFmRNA的表达与食管癌患者的年龄、性别、肿瘤的部位、大小、形状、病理分化程度等临床病理特征无关,与临床分期、远处转移有关。结论:VEGF在食管癌浸润和转移过程中发挥重要作用,检测肿瘤组织中VEGFmRNA水平对预测食管癌的侵袭和转移具有重要的临床意义。

Aim： To investigate the relationship between the expression of vascular endothelial growth factor （VEGF） mRNA and invasion and metastasis of esophageal cancer （EC）. Methods：The primary carcinoma, the corresponding esophageal epithelium tissue in 15 patients with esophageal squamous cell carcinoma （ESCC） were subjected to detect the level of VEGF mRNA using real time RT-PCR and cDNA microarray, respectively. Results ： The expression of VEGF mRNA in 8 （53%） out of 15 patients with ESCC were up-regulated, and the levels were （3.82 ± 0.75） using eDNA chip, and （4.11 ± 0.89） by real time RT-PCR. High level of expression of VEGF mRNA were significantly associated with TNM stages and distant metastases （P 〈 0.01 ） , but not with the sex, age of the patients, and location, size,differentiion degree of EC. Conclusion： VEGF may play an important role in the invasion and metastasis of ESCC. VEGF mRNA assay may be used to predict invasion and metastasis of ESCC.