食管鳞癌分化相关基因表达谱及其染色体精确定位

Cell differentiation correlated genes profile and its accurate location on chromosome in primary esophageal squamous cell carcinoma tissue

基因表达谱,结合临床病理资料筛选食管癌分化相关基因,并在染色体上精确定位。结果:与食管鳞癌分化直接相关的基因有16条,包括MET,KRT4,EGFR,KRT13,CSK,EGR1,CCND1,EPHA2,CEACAM1,PTPN2,HSPBP1,CD-KN1B,BCL2L2,SDC1,CDH1,CTNNA1;主要集中在染色体1p,2p,5q,7p,7q,11q,12p,12q,14q,15q,16q,17q,18p,19q。结论:食管鳞癌发生和演变涉及多条染色体异常的积累。

Aim： To investigate the relationship between chromosome abnormality and cell differentiation in esophageal squamous cell carcinoma （ESCC） tissue. Methods：The genes profiles obtained from 15 cases of ESCC by cDNA microarray were analyzed and located accurately on chromosome. Results： 16 genes had significant correlation with the ESCC differentiation, and they were MET, KRT4, EGFR, KRT13, CSK, EGR1, CCND1, EPHA2, CEACAM1, PTPN2, HSPBP1, CD- KN1B,BCL2L2,SDC1 ,CDH1, and CTNNA1 ,which mostly located on chromosome lp,2p,5q,7p,7q,11q,12p,12q,14q, 15q, 16q, 17q, 18p, and 19q. Conclusion： The accumulation of more than one chromosome abnormality contributes to the carcinogenesis and progression of ESCC.