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A549细胞转染esp1基因后生物学行为的变化 被引量：1

Biological behavior of A549 cells after esp1 gene transfection
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摘要 目的:观察A549细胞转染esp1基因后细胞生物学行为的改变。方法:将IRE-EGFP-esp1质粒用Fu-GENE6脂质体转染到肺腺癌细胞A549,经G418筛选获得转染阳性的细胞系(esp1-A549)。采用DNA荧光染色流式细胞仪分析转染后细胞DNA含量、倍性及细胞周期,计算S期细胞比率(SPF)和DNA指数(DI);采用细胞表面磷脂酰丝氨酸检测法和闪烁计数法分别检测细胞凋亡和增殖情况,同时检测软琼脂集落形成能力。以未转染(A549)、转染空载体(neo-A549)的细胞作对照。结果:与其他2组比较,esp1-A549细胞的DI、SPF及细胞增殖率降低,细胞凋亡率升高。esp1-A549细胞软琼脂集落形成数为3.5±0.6,克隆形成率为12.5%,低于A549细胞的23.3±0.2(t=4.93,P=0.004)和46%。结论:esp1基因的上调表达对A549细胞部分恶性生物学表型具有一定的抑制作用。 Aim： To observe the biological changes of A549 cells after espl gene transfection. Methods： IRE-EGFP-esp1 plasmid was transfected into lung cancer cell line A549 via FUGENE 6 liposome, ploidy, and cell cycle were studied by flow cytometry combined with fluorescent staining,and DNA index （DI） and SPF were calculated. Apoptosis was analyzed by Annexin V-FITC method, and proliferation was detected. Cell colony formation in soft agar was observed as well. Cells transfected IRE-EGFP （neo-A549） and without transfection （A549） were the control. Results： Compared with neo- A549 and A549, DI, SPF, and proliferation of esp1-A549 cells significantly decreased （ P 〈 0. 05 ） , while apoptosis increased（P 〈0.05）. The ability of colony formation in soft agar of esp1-A549 cells was suppressed compared with A549 （ P 〈 0.05 ）. Conclusion ： Up-regulation of espl can inhibit the malignant biological behavior of A549 cells.
作者 刘丽莎 刘昕 刘永康
机构地区 第三军医大学基础医学部分子遗传学教研室 西南医院全军肝胆外科研究所
出处 《郑州大学学报（医学版）》 CAS 北大核心 2006年第5期884-886,共3页 Journal of Zhengzhou University(Medical Sciences)
关键词 esp1基因 A549细胞 肿瘤 凋亡 增殖 克隆形成 细胞周期 DNA倍性 esp1 gene A549 cell neoplasm apoptosis proliferation colony formation cell cycle DNA ploidy
分类号 R394.2 [医药卫生—医学遗传学]
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参考文献10

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共引文献3

同被引文献10

  • 1刘丽莎,刘昕.esp1基因上调表达对A549细胞凋亡影响的研究[J].第三军医大学学报,2006,28(8):828-830. 被引量：1
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郑州大学学报（医学版）
2006年 第5期

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