摘要
目的:建立RP-HPLC测定头孢克肟口含片中头孢克肟含量的方法。方法:色谱柱为ReliaSil C_18(250 mm×4.6 mm, 5μm),流动相为四丁基氢氧化铵溶液(用1.5 mol·L^(-1)磷酸溶液调节pH至7.0)-乙腈(70:30),检测波长254nm。流速0.8 ml·min^(-1)。结果:头孢克肟浓度在2-120μg·ml^(-1)范围内,与峰面积呈良好的线性关系,r=0.999 9;平均回收率为100.6%(n= 9),RSD=1.4%。结论:该方法简便可靠,可用于头孢克肟口含片的质量控制。
Objective: To establish a method for determination the content of cefixime in cefixime buccal tablets by RP-HPLC. Method: The reliasil-C18 column(250 mm × 4.6 mm,5μm) was used, the tetrabutylammonium hydroxide solution (adjust pH to 7.0 with phosphoric acid)-acetonitrile (70: 30) as mobile phase, the detection wavelength was 254 nm, the flow rate was 0.8 μg·ml^-1. Result: The linear range of cefixime was 2 - 120μg·ml^-1, the average recovery was 100.6% ( n = 9 ), RSD = 1.4%. Conclusion: The methods is reliable and accurate, it can be used for quality control the cefixime buccal tablets.
出处
《中国药师》
CAS
2006年第9期800-801,共2页
China Pharmacist