MTS法在磁控加载环境中细胞增殖测定中的应用

APPUCATION OF MTS IN TUMOR CELL PROLIFERATION PROHIBITION STUDIES IN LOADING ENVIRONMENT CONTROLLED BY MAGNETTIC MICROBEADS

以人肝实质细胞癌(hepatocellular carcinoma,HCC)细胞SMCC-7721为研究对象,比较了在磁控加载环境中细胞增殖的3种测定方法。结果表明,由于微球的干扰,台盼蓝法在此环境下的测定容易出现错误统计,同时需时较长。MTT法由于显色物质为非水溶性,在有机溶剂中的再次溶解的均匀度不好掌握,测定结果偏差较大,且容易因溶解过程造成微球漂浮而导致取样点失效。而MTS法在保持测定结果与传统的台盼蓝法无显著差异的前提下,却更为简单快速,特别是能够避免微球对于测定的多种干扰,因此对研究这种加载环境中的细胞增殖,是一种最为适用的方法。

Three methods of cell proliferating measurement were compared with samples of hepatocellular carcinoma （ SMCC-7721 ）. The method using trypan blue was not efficient for large samples and was more incidental in error calculation because of similar shapes of microbeads. As the colorimetric reagent of MTT was not water - soluble, it was difficult to control the uniformity in isopropyl alcohol, which might get the result far from the veracity. Floating of microbeads by violent mixture in the solution also affected the veracity. In comparison, MTS was not only more efficient in cell proliferation study in this loading environment by anti-jamming the effect of microbeads, it also had higher reliability, which showed no observable difference from the traditional method of trypan blue.