摘要
目的探讨5,6-二氯-1-β-D-呋喃核糖苯并咪唑(DRB)对人喉癌Hep-2细胞系增殖、侵袭及凋亡的影响及其作用机制。方法体外培养人喉癌Hep-2细胞,加入不同浓度的DRB,采用MTT法检测Hep-2细胞的增殖情况,流式细胞术观察Hep-2细胞凋亡率的变化,体外侵袭实验观察Hep-2细胞侵袭力的改变。结果DRB可抑制Hep-2细胞的增殖,呈时间和剂量效应关系。流式细胞仪检测二倍体峰前出现凋亡峰,10、20、40、80μmol/L DRB与Hep-2细胞共孵育24 h后的细胞凋亡率分别为8.51%、11.26%、14.99%、25.31%。体外侵袭实验显示,DRB在10μmol/L时即能抑制Hep-2细胞的体外侵袭力,随浓度提高,抑制作用增强。结论DRB能抑制Hep-2细胞增殖,降低细胞侵袭性,诱导细胞凋亡。
Objective To study the effect and mechanism of 5,6-dichloro-1-β-D-ribofuranosyl-benzimidazole （DRB） on pro liferation, apoptosis and invasion of Hep-2 cell line in human laryngeal cancer in vitro. Methods Hep-2 cells cultured in vitro were treated with different concentrations of DRB. Changes of cell proliferation, apoptotic rate and invasion were detected by MTT assay, flow cytometry and matrigel in vitro invasion assay, respectively. Results DRB inhibited the proliferation of Hep- 2 cells in a dose- and time-dependent manner. An apoptosis peak appeared before diploid peak in flow cytometry. After being treated with 10, 20, 40, 80μmol/L DRB, the apoptotic rate in Hel〉2 cells at 24 h was 8. 51 %, 11. 26%, 14. 99% and 25.31%, respectively. The matrigel in vitro invasion assay revealed that DRB began to inhibit the invasion of Hep-2 cells at the concentration of 10μmol/L. With the increase of DRB concentration, the inhibitory effects were increased. Conclusion DRB could inhibit the proliferation, reduce invasion and induce apoptosis of Hep 2 cells.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2006年第4期518-520,524,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
第四届教育部"高校青年教师奖"资助项目
