PCDNA3/p33^(ING1)、wt-p53转染对胃癌细胞株SGC-7901生长抑制及凋亡的影响

Effects of on growth inhibition and apoptosis in gastric cancer cells SGC-7901 transfected by PCDNA3/p33^(ING1) and wt-p53

目的研究p33ING1基因与p53基因间的协同功能对胃癌细胞生长抑制和细胞凋亡的影响。方法构建PCD-NA3/p33ING1真核表达质粒(正义,反义)与wt-p53质粒,将其单、共转染至胃癌细胞株SGC-7901;应用流式细胞仪检测细胞生长周期曲线比例;TUNEL法、MG-P-MY法染色观察SGC-7901的凋亡情况。结果p33ING1单转染和wt-p53共转染的SGC-7901胃癌细胞株较反义组和转染空载体组细胞生长速度减慢,细胞周期G0/G1期延长,S期变短(P<0.05),细胞调亡数增加(P<0.05)。结论p33ING1具有抑癌功能及生物活性,与p53基因共同抑制胃癌细胞的生长,诱导细胞的凋亡,使细胞周期阻滞,二者呈协同依赖关系。

[Objective] To investigate synergistic effects of p33^ING1 and p53 on growth inhibition and apoptosis in gastric cancer cells [Methods] Gastric cancer eeUs SGC-7901 were transfeeted by constructed PCDNA3/p33^ING1 eukaryotie （sense and antisense） expression plasmid and wt-p53 plasmid; the curve ratio of cell cycle phases was analyzed by flow eytometry; the effect of SGC-7901 apoptosis was determined by TUNEL/MG-P-MY combined stain assays. [Results] The study demonstrates that the eell growth rate of SGC-7901 which were transfeeted by p33^ING1 and eotransfeeted by p33^ING1 and wt-p53 was slower than that of PCDNA3 antisense plasmid group and PCDNA3 plain plasmid group; moreover, their phase G0/G1 was longer while phase S was shorter （P 〈0.05）. We also found that the number of apoptosis SGC-7901 cells inereased（P 〈0.05）. [Conclusion] P33 ING1 has the function of cancer growth inhibition, the synergistic effect of p33^ING1 and p53 could inhibit cancer growth, induce apoptosis and block cell cycle phases.